Finally, the mesh quality and complexity were optimized using free software tools Graphite, Meshlab and Meshmixer (Autodesk, San Rafael, CA, USA).Īs a result of the described process we are able to provide three-dimensionally reconstructed, co-registered sets of CBCT scans and micro-slicing colour images of the human temporal bone. Triangulation was performed in 3D Slicer and included moderate smoothing during model creation. The bone segment was additionally exported as a voxel model as per the requirements of surgical simulators. Segmented volumes were converted to three-dimensional triangulated surface models of all anatomical structures.